PrimeEditing
Prime Editing Template Generator (RTT/PBS)
Break down RTT and PBS logic with a purpose-built template builder.
Build RTT and PBS templates with stability guardrails and mismatch previews in Omnis Helix, the Genome IDE for prime editing design.
Tool definition
What the tool is
The Template Generator visualizes RTT and PBS segments, showing exactly where mismatches and edits sit relative to the nick.
Decision pressure
Why scientists care
RTT/PBS selection is often trial-and-error, leading to unstable priming or edits outside the window.
Genome IDE fit
How the Genome IDE helps
Interactive RTT/PBS sliders with guardrails for GC%, Tm, and length
Scoring model
How the algorithm works
Templates are scored on priming stability, mismatch placement, and proximity to the nick site.
Evaluation path
Try it in the Genome IDE
Load your desired edit, slide RTT/PBS lengths, and lock in the template with the highest stability badge.
Questions
FAQ
What RTT/PBS ranges do you support?
Common ranges (8–20 nt PBS, 10–40 nt RTT) are supported with custom overrides when needed.
Can I preview secondary structure?
We flag strong hairpins or homopolymers and suggest alternates before export.
Do you handle reverse complements?
Yes—templates are oriented automatically based on target strand and nick direction.