PrimeEditing
Prime Editing pegRNA Designer
pegRNA design tool with RTT/PBS pairing and edit evidence generation.
Plan pegRNAs for prime editing with RTT and PBS design, validation, and context visualization inside Omnis Helix, a modern Genome IDE for genome engineering.
Tool definition
What the tool is
The pegRNA Designer turns desired edits into validated pegRNAs with RTT/PBS pairs sized to the change you want to introduce.
Decision pressure
Why scientists care
Prime editing design often lives in spreadsheets with manual RTT/PBS tweaks and risky copy/paste steps.
Genome IDE fit
How the Genome IDE helps
Automatic RTT/PBS sizing based on edit distance and GC balance
Scoring model
How the algorithm works
RTT/PBS candidates are enumerated with length sweeps, GC constraints, and melting estimates, then ranked by model implied priming stability.
Evaluation path
Try it in the Genome IDE
Paste reference + edited sequences, generate pegRNAs, and review QC badges before exporting.
Questions
FAQ
Do you support PE2, PE3, and PE5 setups?
Yes—choose the strategy and Helix proposes compatible nicking guides and offsets.
Can I fix RTT or PBS lengths?
Lock either length and Helix optimizes the other while keeping stability and edit reach in range.
Do you model indel byproducts?
We simulate likely outcomes and show model implied spectra (assumption bound) next to each pegRNA.