PrimeEditing
pegRNA Checker & Validator
QC pegRNAs before synthesis: length, stability, and off-target context.
QC pegRNAs for length, stability, nick alignment, and off-target context before synthesis inside Omnis Helix, the Genome IDE for Prime Editing.
Tool definition
What the tool is
The pegRNA Checker applies a QC gauntlet to every candidate—flagging stability risks, off-target liabilities, and design rule violations.
Decision pressure
Why scientists care
Prime editing failures often trace back to small template or nicking misalignments caught too late.
Genome IDE fit
How the Genome IDE helps
Length, GC, Tm, and secondary-structure checks with severity tiers
Scoring model
How the algorithm works
QC rules blend thermodynamic models, heuristics from published PE datasets, and Helix’s off-target scoring.
Evaluation path
Try it in the Genome IDE
Import pegRNAs or generate them in Helix, run validation, and fix any flagged items instantly.
Questions
FAQ
Can I set custom QC thresholds?
Yes—tune GC/Tm ranges, allowed offsets, and structural thresholds per project.
Do you validate nicking guide strands?
We ensure nicking sgRNAs are on the correct strand and distance for PE3/PE5 strategies.
Are QC results exportable?
Export PDF/CSV summaries or keep them in Helix for traceability.