Utilities
HDR ssODN Donor Template Builder
Build ssODN donors with homology arms, PAM disruption, and QC in one pass.
Generate HDR ssODN donors with tuned homology arms and PAM-disrupting mutations in Omnis Helix, the Genome IDE for CRISPR teams.
Tool definition
What the tool is
The ssODN Donor Builder generates single-stranded donors aligned to your CRISPR cut, with configurable arms and protective silent mutations.
Decision pressure
Why scientists care
Manual donor drafting risks strand flips, weak arms, or forgotten PAM blockers.
Genome IDE fit
How the Genome IDE helps
Adjust left/right homology arm lengths with GC/Tm guardrails
Scoring model
How the algorithm works
Arm lengths are optimized for balance and stability; GC and repeat content are checked automatically.
Evaluation path
Try it in the Genome IDE
Select your guide, set arm targets, and add optional PAM blockers, then export the donor as FASTA/CSV/PDF.
Questions
FAQ
Do you support biotin or phosphorothioate mods?
You can add common modifications and Helix will include them in the order notes.
What arm lengths work best?
Defaults start at 30–60 nt; sliders and guardrails help you adjust for your system.
Can I lock strand orientation?
Yes—choose sense/antisense and Helix keeps the orientation consistent across exports.