CRISPR
CRISPR Guide RNA Designer
Design high-fidelity CRISPR gRNAs using Omnis Helix’s evidence driven engine.
Design high-fidelity CRISPR guide RNAs with genomic context, off-target aware workflows, and evidence generation inside Omnis Helix, the first Genome IDE.
Tool definition
What the tool is
The CRISPR Guide RNA Designer is a focused surface for drafting, validating, and packaging gRNAs inside the Genome IDE.
Decision pressure
Why scientists care
Guide design normally lives across spreadsheets, web calculators, and command-line aligners—context is lost and errors creep in.
Genome IDE fit
How the Genome IDE helps
Live GC%, melting, and on/off-target heuristics with side-by-side genome context
Scoring model
How the algorithm works
Guides are scored with hybrid heuristics: GC balance, seed stability, model implied cutsite accessibility, and off-target proximity to coding exons.
Evaluation path
Try it in the Genome IDE
Load a locus, sweep the PAM track, and drop candidate guides directly into Omnis Helix.
Questions
FAQ
Which nucleases are supported?
SpCas9, SaCas9, Cas12a/Cpf1, and high-fidelity variants with their PAM rules baked in.
Can I keep cloning overhangs attached?
Yes—Helix appends overhangs, U6 promoters, or adapters while preserving the core spacer for scoring.
How do I export to other tools?
Export CSV/FASTQ/GenBank or handoff directly into Helix’s evidence generation and analysis steps.