CRISPR
CRISPR Multiplex Planning Tool
Simulate interactions between multiple gRNAs before you build the construct.
Plan multiplex CRISPR designs, detect guide collisions, and model combined off-target risk in Omnis Helix, a Genome IDE built for complex edits.
Tool definition
What the tool is
The Multiplex Planning Tool orchestrates guide sets, checking distance, frame impacts, and shared off-target liabilities as you build constructs.
Decision pressure
Why scientists care
Multiplex designs explode in complexity—guide collisions, shared off-targets, and delivery constraints derail experiments.
Genome IDE fit
How the Genome IDE helps
Visual lane for each guide with distance checks and frame awareness
Scoring model
How the algorithm works
Guides are simulated together with additive risk scoring and collision detection for overlapping PAMs or spacers.
Evaluation path
Try it in the Genome IDE
Drop your target region, stack guides, and watch Helix auto-check spacing and conflicts.
Questions
FAQ
How many guides can I plan at once?
We support typical small pools (2–20 guides) with performance headroom for more depending on sequence length.
Can I mix nucleases?
Yes—define per-guide nuclease settings and Helix applies the right PAM and cut rules.
Do you model delivery constraints?
We include promoter/terminator footprints and payload size estimates to keep constructs feasible.